Nanoparticle-Mediated Genetic Engineering of Plants

Genetic Engineering of Plants

Contents 1. Introduction 2. Transformation of dicotyledonous plants 2.1 Transformation using Agrobacterium tumefaciens 2.1.1 The role of A. tumefaciens chromosomally encoded proteins and the Vir proteins 2.2 Other transformation methods 3. Transformation of monocotyledonous plants 3.1 Biolistic transformation 3.1.1 Gene silencing 3.2 Agrobacterium transformation 4. Transformation of algae 5. Pr...

Genetic engineering of salinity-tolerant plants

olecular techniques such as recombinant DNA technology may ultimately have their most significant effect on agriculture. Recent advances raise the possibility of the development of new plant germplasm through the introduction of any gene from any organism into plants. Several leading laboratories have achieved the transfer and expression of bacterial and foreign plant genes in plant cells. Incr...

Genetic Engineering of Tomato Plants Expressing β-Glucuronidase through Agrobacterium-mediated Transformation

Genetic Engineering of Plants for Resistance to Viruses

Genetic engineering has been identified as one key approach to increasing agricultural production and reducing losses due to biotic and abiotic stresses in the field and in storage (Sairam and Prakash, 2005; Yuan et. al., 2011). This chapter primarily deals with resistance to viral diseases. It is therefore very important that anyone embarking on a research project to genetically engineer plant...

Toward stable genetic engineering of human O-glycosylation in plants.

Glycosylation is the most abundant and complex posttranslational modification to be considered for recombinant production of therapeutic proteins. Mucin-type (N-acetylgalactosamine [GalNAc]-type) O-glycosylation is found in eumetazoan cells but absent in plants and yeast, making these cell types an obvious choice for de novo engineering of this O-glycosylation pathway. We previously showed that...